Relative mitochondrial polarization was expressed as the ratio of emission at 590 nm to that at 530 nm (Shin et al. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThe absorbance was recorded on a spectra Max i3x microplate reader (Molecular Devices, USA) at 450 nm. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. Molecular Devices are known for both the reliability and the wide uses of their products. 2C). It allows you to explore cellular . Because a PMT needs to be set to a specified gain to reach a specific sensitivity,Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. Besides, it’s possible to examine each page of the guide singly by using the scroll bar. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. See more, and do more, with the SpectraMax® i3/i3x Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick imaging and analysis of cells and gives you a front-row view of phenotypic changes that accompany cytotoxicity,. Patented user-exchangeable cartridge design expands the system's detection capability making it. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The treatments were. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . 吸収度マイクロプレートリーダー SpectraMax® Mini. Remove the transport bracket and store the retaining screws in the bag that held the 3. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 1 1 10 100 e e e e e e eThe SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Analysis of HTRF assays involves Cisbio’s patented ratiometric reduction method based on the two emission wavelengths detected. Nuclear Receptor. Prime function 20 amol. The NET-specificity of the detected signal was determined by incubation with DNase I (50 U/mL, Qiagen, Germany). . No Stain Analysis: Eliminate cell staining for cell counting and confluence measurements using. 0´ 104 6. 304 signal level saturation. The SpectraMax ® i3x. Table 1. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. ) wide and 65 cm (25. no. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthTNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. Register to download your free eBook today. 4. An MPO assay has been performed to quantify the inflammation reduced by the GAR-PLGA-ES100 NPs in the inflamed cells . Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. 9 x 10-15. The fluorescence spectra was obtained on a Spectra Max i3x Multi-Mode Detection Platform. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Check software compatibility with Windows 10 and locate your software installer. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Cell counters for accurate and. Get Pricing. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesThe SpectraMax i3x reader features a cooled photomultiplier tube (PMT) for low background noise in luminescence. The TRF intensities measured are proportional to the level of extracellular acidification in each sample. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. SoftMax Pro Software enables users to quickly set up analysis of cell counts via a simple point and click user interface. 3. SoftMax Pro. Prepare your iD series instrument for shipment. 305 reference level saturation. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Replicate values were averaged and imported into Design-Expert 8. SpectraMax i3x plate reader (Molecular Device, contact supplier directly) Vacuum pump system (e. 3 In this application note we demonstrate a linear dynamic range of 6 decades for recombinant firefly and Renilla luciferases, as well as linear detection of luciferase-transfected. Carbonyl assay The plates for carbonyl detection were prepared as described. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Use the 3. 12 mg/mL) of polysaccharides were prepared after addition of glucosum anhydricum for calibration. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologySpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Explore a wealth of applications in one future-ready system that measures spectral-based absorbance, fluorescence, and luminescence. The turbidity. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Align the two slots on the bottle holder with the two pegs on the rail. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. Get a front row view of your cells. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. Fluorescence emission spectra were collected on an ELISA molecular system (Spectra Max i3x). In this product video, see how the SpectraMax M5 quickly reads samples. 11. SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax MiniMax 300 Imaging Cytometer *Here, the NucView 488 with DMSO formulation was used. 0´ 104 1. The MPO assay was conducted according to the. 2. +44 118 944 8000. Table 1. Nucleic Acid and Protein Quantitation. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. Downloads. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. Prepare your iD series instrument for shipment. 3: The cuvette chamber. The chamber. Detection cartridge modularity allows for easy addition of new read modes within minutes without. 0´ 104 8. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. 1. Molecular Devices’ ScanLater Western Blot Detection Cartridge uses TRF detection to turn its SpectraMax i3X and Paradigm plate readers into Western blot readers. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. ISM Equipment Classification (Group 1, Class A) This equipment is. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthCapture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. The SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. Also refer to this Multimode Reader Comparison Chart. pathways and protein activation and expression in one system. Besides, it’s possible to examine each page of the guide singly by using the scroll bar. Published Dec 16, 2020 | Updated Oct 10, 2023. Microplate assays were performed on a multimode microplate detector (Molecular Devices Spectra Max i3x). Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology:. We would like to show you a description here but the site won’t allow us. Promega. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. Contact our support team with your questions regarding Molecular Devices products and applications. Average 98 stars, based on 1 article reviews. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Fluorescence at 365 nm excitation and 450 nm emission filters was determined based on a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA). The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. Microplate optimization should be performed the first time the assay is run, and subsequently for . Analysis. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). 0. each new lot of plates, to ensure that the center of each well is read. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. The SoftMax Pro Software provides integrated instrument control. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). *On the SpectraMax i3x reader only. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. 01 0. 3, were mixed in a final volume of 100 μL in 10 mM sodium phosphate buffer, pH 7. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects and changing objectives, the i Series has you covered. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthThe SpectraMax i3x reader grows with you as your research areas expand. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Reaction catalyzed by the firefly luciferase. 0. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Example: [Drawer]. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. 1 mg/mL density of resazurin, protected of the light, incubated for 1 h at room temperature. Order Now. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks. Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. Download PDF. The cell imaging was captured on Zeiss LSM 980 confocal microscopy. The UV-Vis spectroscopy was measured on a TU-1900Beijing PuXi instrument. Increased fluorescence performance. 0 mm hex key. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . com. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. Budget-friendly multi-mode microplate reader . Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. Apoptosis was measured by the Annexin V-FITC/PI Apoptosis Detection Kit. 7 mm for all shake intensities. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataThe SpectraMax i3x reader and MiniMax cytometer can be used with FluoroBlok cell culture inserts to directly count cells from high-quality images to measure cellular migration over time. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Figure 1. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Turbidity measurements were performed with a Spectra Max® i3x microplate reader to study the effect of oxidation on the kinetics of sFN formation. Anne T. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Winnersh Triangle. Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. Figure 2-23: Bottle holder To install the bottle holder: 1. Multimode Reader Comparison Chart. 312 gain calibration failed. 58% for the detection module and 2. The plate was covered and incubated for one hour at room temperature. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax Microplate Readers. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Introduction. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely low light. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeSpectraMax MiniMax 300 イメージングサイトメーター は、研究室でのアップグレードが可能なSpectraMax i3xシステム専用のオプションで、ユーザーは複雑なイメージングシステムに投資することなく、細胞増殖を解析できます。SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. 価格、見積もり、お近くの販売店を. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. polarization performance of the FilterMax®, SpectraMax® Paradigm®, and select cartridges or read modes of the SpectraMax i3, i3x, and iD5 readers. Home > Search Results > Danaher Inc > spectramax i3x. Register to download your free eBook today. Download PDF. Pick the best speed, sensitivity, and price for your needs. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. The SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer is equipped with transmitted light (TL), green fluorescence (Ex/Em: 460/541), and red (625/713) fluorescence channels. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization options in the instrument settings in SoftMax Pro Software). i3x. Eliminate time-dependent substrate addition steps. ) deep, preferably with access on all sides. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Molecular Devicesの全商品を見る. 3: The cuvette chamber. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. Every SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. Quantification of NETs was performed with the SpectraMax i3x microplate-reader (Molecular Devices, United Kingdom). The SpectraMax ® i3x reader provides the. Increased fluorescence performance. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. spectramax i3x (Danaher Inc)The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Sensitive TRF detection of two simple, mix-and-measure assay workflows. Microplate Stacker. Examples: fiPress carefully to engage,fl fiDo not press down. 313 reference gain check fail. 2 x 10-14. SpectraMax i3Instrumenttovisuallyinspectyoursampleandtoruncell-basedassaysat cellularorwhole-cellresolution. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. The absorbance at 450 nm was read with a Spectra Max i3X microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA), and the data at 30–40 min post-treatment were analyzed. This way you’ll save time on finding the necessary info. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. Time-. We would like to show you a description here but the site won’t allow us. Eliminate time-dependent substrate addition steps. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 303 unable to cal dark current. 0. Flow cytometry was used to measure ROS concentrations, based on fluorescence intensity (FL-1, 530 nm) of 10,000 cells. Item SpectraMax i3x Multi-Mode Microplate Readers; Company Molecular Devices LLC; Catalog Number SpectraMax i3x <Disabled> This product is no longer available on Biocompare. The plate was shaken and incubated in the dark for 10 minutes. The SpectraMax ® i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. To learn more about the device, please watch this short video. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Find regional support. See5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideMolecular Interactions. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Fluorescence Microplate Reader Comparison Chart. Louis, MO) and used as received without further purification. Unpacking and setting up the instrument includes the following procedures: • Unpacking the instrument and saving the original packaging. 22 μ m fi ltration unit (Sigma, cat. 0 | 9 reviews | Write your own review Measures Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgradesMolecular Devices SpectraMax i3x Multi-Mode Microplate Platform. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Cet appareil ISM est confomre à la norme NMB-001 du Canada. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. com Check our website for a current listing of worldwide distributors. Spectramax I3x, supplied by Danaher Inc, used in various techniques. See SpectraMax® Microplate Reader User Manual Download Page. The standard solutions (0. VAL014, right table) assays on the SpectraMax i3x reader are shown below. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. 58% for the detection module and 2. Description 1. The CellTiter-Glo assay from Promega uses luciferase. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. 635. The information below applies to a standard 96 well plate, set to a linear shake mode. They were allowed to attach and grow overnight in a 37°C, 5% CO 2. Italic and boldface type are used for emphasis. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable. 5. Fluorescence Reader. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Synthesis of NORP Synthesis of compound 1. 312 gain calibration failed. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. Controls containing all reaction components with either DI . ). The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. each new assay. 1321N1, a human astrocytoma cell line. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Find regional support. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. Figure 1. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. Red and green fluorescence was then measured (emission at 590 and 530 nm, respectively, with excitation at 490 nm) using a fluorescence microplate reader (Spectra Max i3x, Molecular Devices Inc. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. TUNE Tunable Wavelength Detection Cartridge. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. 8 Antibodies and. As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. The SpectraMax i2X is used for cell migration, cell. Structured Review. Home > Search Results > Danaher Inc > spectramax i3x. *On the SpectraMax i3x reader only. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. i3. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. User-upgradeable applications The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,i3x Multi-Mode Microplate Reader. Simplified workflow: Add cellular imaging assays in a small footprint. In dual reporter gene assays, cells are transfected with two vectors, the first containing an experimental reporter gene coupled to a regulated promoter of interest, andSpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. Generate more data points without the need to dilute. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. The fluorescence spectra were acquired using a microplate reader (Spectra Max i3x; Molecular Devices, USA). High resolution X-ray photoelectron spectroscopy (XPS) of cCQD was obtained on a K-Alpha+ (Thermo Fisher Scientific, Massachusetts, USA) spectrometer. Pick the best speed, sensitivity, and price for your needs. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Molecular Devices Software. 001 0. 3. Spectramax I3x, supplied by Danaher Inc, used in various techniques. Unlock your i3/i3x instrument after shipment. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. 5 decades, with sensitive detection of cells. Measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. SpectraMax i3x Multi-Mode Detection Platform User Guide. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Prismatic colour and motion for everyone. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. 모든 제품. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Lower limits of detection for ATP determined using the ATPlite . 2 (Molecular Devices, San Jose, CA, USA). Afterward, the absorbance was measured at 450 nm on a Microplate Reader (Spectra Max i3x, Molecular Devices, USA). Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Introduction. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. o For example: Ex: 485/Em 525/Cutoff 515 4. SoftMax Pro GxP Compliance Software. 0001 0. 2 in. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. • SpectraMax i3x • Onboard luminescence • Detection cartridge (LUM 96) • SpectraMax iD5 • SpectraMax L • SpectraMax M5e • SpectraMax Mini. As shown in the results above, the DELFIA cell proliferation assay combined with the SpectraMax i3 reader gives researchers a very sensitive test for measuring cell proliferation. Supplier: Molecular Devices. The SpectraMax i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6 - 384 well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. Published Dec 16, 2020 | Updated Dec 16, 2020. Spectranet customer portal The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. 제품. Spectramax I3x, supplied by Danaher Inc, used in various techniques. Specifically, we present neurite. The plate was shaken and incubated in the dark for 10 minutes. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Plate chamber design of the SpectraMax category 1 microplate readers. The dynamic range of the assay is about 3. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® Microplate Readers and SoftMax® Pro Software. Dependingontheapplication,theinstrumentcanread6,12,24,48,96,and384-well microplates. For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. As. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. HeLa cells were plated at 15,000 cells per well in 100 µL of medium in a 96-well black, clear-bottom microplate. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength.